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goscript reverse transcription (rt) equipment  (Promega)

 
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    Structured Review

    Promega goscript reverse transcription (rt) equipment
    Goscript Reverse Transcription (Rt) Equipment, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goscript+reverse-transcription+(rt)+system/goscript+reverse+transcription+system/pm40646181-124-1-6
    Average 90 stars, based on 1 article reviews
    goscript reverse transcription (rt) equipment - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: LUCAT1 Activates the Malignant Phenotypes of Lung Cancer Cells via Regulating P53 Ubiquitination
    Article Snippet: .. GoScript Reverse Transcription (RT) System (Promega) kit and GoTaq qPCR master mix were used for reverse transcription. .. The primers were shown in , and the reaction was put in effect with the instructions with Step One Plus Fluorescence Quantitative PCR (ABI) instrument.

    Article Title: An engineered tumor organoid model reveals cellular identity and signaling trajectories underlying SFPQ-TFE3 driven translocation RCC
    Article Snippet: Total RNA was extracted using Trizol reagent (Invitrogen) or using the Macherey-Nagel RNA isolation kit according to manufacturer’s instructions. .. Extracted total RNA was used to produce cDNA using the GoScript reverse transcriptase system (Promega) and qRT-PCR was subsequently performed using IQ SYBR green mix (Bio-Rad) according to the manufacturer’s instructions. ..

    Article Title: Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade Therapy
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoDrop spectrophotometer, with an A260/A280 ratio exceeding 1.9 considered satisfactory. .. Subsequently, the cDNA was amplified from 2 μg RNA using a RT-PCR kit (GoScript Reverse Transcription system, Promega, A5001). cDNA was then used as a template to perform qRT-PCR analysis. .. Quantitative PCR was conducted utilizing the GoTaq qPCR Master Mix, 2� (Promega, A600A), and the samples were analyzed on a 7500 Real Time PCR System (Applied Biosystems).

    Article Title: Potential cellular targets of platinum in the freshwater microalgae Chlamydomonas reinhardtii and Nitzschia palea revealed by transcriptomics.
    Article Snippet: Platinum group element levels have increased in natural aquatic environments in the last few decades, in particular as a consequence of the use of automobile catalytic converters on a global scale.. Concentrations of Pt over tens of μg L have been observed in rivers and effluents.. This raises questions regarding its possible impacts on aquatic ecosystems, as Pt natural background concentrations are extremely low to undetectable.

    Real-time Polymerase Chain Reaction:

    Article Title: LUCAT1 Activates the Malignant Phenotypes of Lung Cancer Cells via Regulating P53 Ubiquitination
    Article Snippet: .. GoScript Reverse Transcription (RT) System (Promega) kit and GoTaq qPCR master mix were used for reverse transcription. .. The primers were shown in , and the reaction was put in effect with the instructions with Step One Plus Fluorescence Quantitative PCR (ABI) instrument.

    Quantitative RT-PCR:

    Article Title: An engineered tumor organoid model reveals cellular identity and signaling trajectories underlying SFPQ-TFE3 driven translocation RCC
    Article Snippet: Total RNA was extracted using Trizol reagent (Invitrogen) or using the Macherey-Nagel RNA isolation kit according to manufacturer’s instructions. .. Extracted total RNA was used to produce cDNA using the GoScript reverse transcriptase system (Promega) and qRT-PCR was subsequently performed using IQ SYBR green mix (Bio-Rad) according to the manufacturer’s instructions. ..

    Article Title: Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade Therapy
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoDrop spectrophotometer, with an A260/A280 ratio exceeding 1.9 considered satisfactory. .. Subsequently, the cDNA was amplified from 2 μg RNA using a RT-PCR kit (GoScript Reverse Transcription system, Promega, A5001). cDNA was then used as a template to perform qRT-PCR analysis. .. Quantitative PCR was conducted utilizing the GoTaq qPCR Master Mix, 2� (Promega, A600A), and the samples were analyzed on a 7500 Real Time PCR System (Applied Biosystems).

    SYBR Green Assay:

    Article Title: An engineered tumor organoid model reveals cellular identity and signaling trajectories underlying SFPQ-TFE3 driven translocation RCC
    Article Snippet: Total RNA was extracted using Trizol reagent (Invitrogen) or using the Macherey-Nagel RNA isolation kit according to manufacturer’s instructions. .. Extracted total RNA was used to produce cDNA using the GoScript reverse transcriptase system (Promega) and qRT-PCR was subsequently performed using IQ SYBR green mix (Bio-Rad) according to the manufacturer’s instructions. ..

    other:

    Article Title: Comprehensive pan-cancer analysis indicates key gene of p53-independent apoptosis is a novel biomarker for clinical application and chemotherapy in colorectal cancer
    Article Snippet: Two micrograms of RNA were used as a template and reverse transcribed to complementary DNA (cDNA) using a GoScript Reverse Transcription (RT) System (Promega, Madison, WI, USA) according to the manufacturer’s instructions.

    Article Title: Sex-specific Stone-forming Phenotype in Mice During Hypercalciuria/Urine Alkalinization.
    Article Snippet: Sex differences in kidney stone formation are well known.. Females generally have slightly acidic blood and higher urine pH when compared with males, which makes them more vulnerable to calcium stone formation, yet the mechanism is still unclear.. We aimed to examine the role of sex in stone formation during hypercalciuria and urine alkalinization through acetazolamide and calcium gluconate supplementation, respectively, for 4 weeks in wild-type (WT) and moderately hypercalciuric [TRPC3 knockout [KO]( / )] male and female mice.

    Article Title: Acting mechanism and clinical significance of hsa_circ_0005927 in the invasion and metastasis of gastric cancer.
    Article Snippet: Then, reverse transcription was performed to generate cDNA using the GoScript Reverse Transcription (RT) System (Promega, Madison, WI, USA).

    Article Title: Cancer marker TNFRSF1A: From single‑cell heterogeneity of renal cell carcinoma to functional validation
    Article Snippet: RT was carried out using a RT kit (Promega GoScriptTM Reverse Transcription System; Thermo Fisher Scientific.

    Amplification:

    Article Title: Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade Therapy
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoDrop spectrophotometer, with an A260/A280 ratio exceeding 1.9 considered satisfactory. .. Subsequently, the cDNA was amplified from 2 μg RNA using a RT-PCR kit (GoScript Reverse Transcription system, Promega, A5001). cDNA was then used as a template to perform qRT-PCR analysis. .. Quantitative PCR was conducted utilizing the GoTaq qPCR Master Mix, 2� (Promega, A600A), and the samples were analyzed on a 7500 Real Time PCR System (Applied Biosystems).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Blocking WNT7A Enhances MHC-I Antigen Presentation and Enhances the Effectiveness of Immune Checkpoint Blockade Therapy
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoDrop spectrophotometer, with an A260/A280 ratio exceeding 1.9 considered satisfactory. .. Subsequently, the cDNA was amplified from 2 μg RNA using a RT-PCR kit (GoScript Reverse Transcription system, Promega, A5001). cDNA was then used as a template to perform qRT-PCR analysis. .. Quantitative PCR was conducted utilizing the GoTaq qPCR Master Mix, 2� (Promega, A600A), and the samples were analyzed on a 7500 Real Time PCR System (Applied Biosystems).



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